Automating HPLC Method Development, Robustness Testing & Validation
Duration: 1 hour and 10 minutes
Basic principles and approaches regarding HPLC method development are presented including preliminary considerations, running initial scouting runs, procedures for setting up a series of experiments (a survey), and valuable pointers on evaluating the results. Throughout, the key optimization parameters are discussed and evaluated.
Based on the initial results, suggestions are provided for follow-up experiments, including interpolation routines. These are intended to help gain additional information and possible improvements to the method.
Finally, strategies for method robustness testing are discussed as a part of initial method validation.
HPLC Troubleshooting (Part 2 of 2*): Recognizing diagnostic clues and interpreting chromatographic results
Duration: 117 minutes
Basic troubleshooting strategies will be applied, covering all aspects of an HPLC system. This will include discussions on how to best use all your clues in order to isolate the problem(s). Clues from instrument components, chromatograms and results will be explored. Also, various symptoms are related to possible HPLC problems and the probable causes of these problems are explored. Example data/results are diagnosed to demonstrate and promote good troubleshooting skills.
* Attending Part 1 is helpful, but not necessary.
Maintaining Day-to-Day Performance and 24/7 Reliability on Your HPLC
Find out from skilled scientists how to achieve top-level performance on your HPLC system today, tomorrow and for years to come. Participants of the archived online seminar will learn the primary performance expectations for an HPLC, highlighted via corresponding proof statements and recommended ways to help maintain reliable results. The archived seminar is approximately 1 hour and ten minutes in length and can be accessed at your convenience.
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